A ready-to-use one step immune cell differentiation medium

PredictCan Biotechnologies has developed OneSmartDiff, a ready-to-use one-step cell differentiation medium to facilitate and to improve your research results.

OneSmartDiff medium allows to differentiate monocytes or PBMCs into activated macrophages and dendritic cells in ONLY ONE step. No need for tedious and expensive steps!


  • Ready-to-use cell differentiation medium for a one-step differentiation of monocytes to activated macrophages and dendritic cell populations

  • Easy and low-cost production of activated macrophage and dendritic cell population

  • Study immune cells differentiation and function under physiological conditions

Production of activated monocyte-derived macrophages and dendritic cells

THP-1 monocytes were cultured for 6 days in OneSmartDiff medium. Monocyte-derived macrophages and dendritic cells were quantified based on their shapes.

Drug-induced cytokine expression in monocyte-derived macrophages and dendritic cells

Monocyte-derived macrophages & dendritic cells were prepared with OneSmartDiff medium and then treated with troglitazone. Cytokine expression was quantified by qPCR. Results are shown as fold change to untreated condition.